Method And Materials For A Light Source Filter Lens

Method And Materialsa Light Source Filter Lens Lens

Method and materials A light source, filter lens, two polarization lenses, four Translation Stage, 40x objective lens(blue), 10x objective lens(yellow), a camera with a USB cable, sample made of gold, At first, we installed the light source (green laser)then respectively we fixed the filter lens and then the 40x objective lens and in the opposite way the 10x objective lens, and on the same line, so that the distance between the 40x objective lens and the 10x objective lens is the focal length of each lens. they fixed In the direction of the camera, we set the 40x objective lens. On the two Translation Stage, so that we get the dimension of x-axes and y-axes, and then we installed the golden sample on a Translation Stage in the middle between the 40x and 10x and is moved horizontally and vertically. In the beginning, we used the green laser so that we could get a more accurate measurement and the materials would be in a straight line, then we replaced it with white light Result when we used the microscope, we have detected some holes.

Paper For Above instruction

Research in optical microscopy often involves precise methodology and the selection of appropriate materials to examine micro-scale samples. In our experiment, we utilized a comprehensive setup that includes a versatile light source, various lenses, and meticulous sample positioning to analyze a gold specimen effectively. This study underscores the importance of accurate alignment, suitable illumination, and adaptable equipment configurations to achieve reliable imaging and measurement results.

The method primarily involved establishing a controlled light environment to observe the gold sample under different illumination conditions. Initially, a green laser served as the light source due to its coherent output and high precision, facilitating exact measurements and proper alignment of the optical components. The green laser’s collimated beam ensured that the sample and lenses remained in a straight, aligned configuration, which is crucial for high-resolution microscopy.

Subsequently, the optical setup was assembled by fixing the filter lens and the 40x objective lens along the same optical axis, ensuring that the distance between the 40x and 10x objective lenses corresponded precisely to their respective focal lengths. This alignment provides optimal focus and clarity when magnifying the gold sample. The 10x objective lens was positioned opposite the 40x lens, and both were secured in a manner that maintained their fixed position along the optical path. The camera was strategically aligned with the 40x lens to capture clear, high-resolution images of the sample.

To facilitate precise sample positioning, four Translation Stages were employed. The two stages along the X and Y axes allowed for meticulous movement of the gold sample in multiple directions, enabling detailed examination of specific areas of interest. The gold sample was mounted in the middle of the setup on its own Translation Stage, which could be moved horizontally and vertically. This flexibility ensured that specific features, such as the detected holes in the sample, could be thoroughly analyzed through controlled displacements under the microscope.

The choice of light source was initially a green laser, which provided a highly collimated and monochromatic beam ideal for accurate measurements and alignment. Using the green laser minimized aberrations and enhanced the clarity of observed features, particularly the holes and defects in the gold sample. After establishing and documenting measurements under laser illumination, the light source was replaced with white light to emulate more typical illumination conditions in standard microscopy. This transition was necessary to observe how the sample's features appeared under broader spectral illumination, which is more representative of practical applications.

The visual observations made during the experiment revealed that the gold sample contained some holes, which were detectable under the optical microscope. The high magnification afforded by the 40x objective lens allowed for detailed visualization of these features. The detection of holes underscores the importance of suitable light sources, precise alignment, and adjustable instrumentation to accurately analyze microstructural features in metallic samples. Such features are crucial in assessing the quality and integrity of the material, especially in fields like materials science and nanotechnology.

This experimental framework demonstrates the significance of integrating multiple optical components—such as filters, polarization lenses, and translation stages—to achieve accurate microscopy results. The careful setup of these elements ensures that the observations are both precise and repeatable. Moreover, the use of different illumination sources (laser versus white light) offers insights into how various lighting conditions influence the visibility and resolution of microstructural features. In conclusion, meticulous arrangement and control of optical parameters are essential in optical microscopy to facilitate detailed, reliable analysis of small-scale materials like gold with micro-defects such as holes.

References

• Born, M., & Wolf, E. (1999). Principles of Optics: Electromagnetic Theory of Propagation, Interference and Diffraction of Light. Cambridge University Press.