Read Chromatogram Comparisons Experiment Perform The Analysi

Read Chromatogram Comparisons Experimentperformthe Analysis Procedure

Read Chromatogram Comparisons Experimentperformthe Analysis Procedure. Read Chromatogram Comparisons Experimentperform the Analysis Procedure. Read Chromatogram Comparisons Experiment Perform the analysis procedure on two different brands of black felt tip pens and compare the two paper chromatograms. Choose one of the following assignment options: Assignment A Take photos of your experiment showing the different color bands produced in the two paper chromatograms. Submit photos of your experiment in an email to your instructor (provided in your course syllabus). You may take digital photos of the chromatograms or scan a copy of the chromatograms and email as an attachment to your instructor. Assignment B Write a word paper describing the experiment. Include a description of all color bands produced in the two paper chromatograms with the order of colors that were separated out. Do the number, color and sequence of colored bands correspond between the two paper chromatograms or do they differ? What are the differences?

Paper For Above instruction

The purpose of this experiment is to understand the process of paper chromatography by analyzing and comparing the chromatograms produced by two different brands of black felt tip pens. Paper chromatography is an analytical technique used to separate and identify mixtures of different compounds based on their movement through a stationary phase (the paper) under the influence of a solvent (the eluent). This experiment demonstrates how different ink formulations, even within the same color such as black, can vary in their component compounds, leading to distinguishable color bands on the chromatogram.

The materials utilized for this experiment include two different brands of black felt tip pens, chromatography paper (or absorbent filter paper), a suitable solvent such as water or alcohol-based solution, a ruler, a pencil, and a camera or scanner for documenting results. The procedure involves drawing a small dot of ink from each pen at the bottom of the chromatography paper, ensuring that the ink is above the baseline to prevent smearing. The paper is then suspended in a container with a solvent that rises up through capillary action, carrying the pigments with it.

As the solvent ascends, different components of the ink dissolve and migrate at varying rates, resulting in a separation of colors along the length of the paper. Once the solvent front nears the top of the paper, the experiment is concluded, and the chromatogram is allowed to dry. The resulting color bands are then observed, measured, and documented with photographs or scanned images for analysis.

In analyzing the chromatograms of each brand, attention is paid to the number of distinct color bands, their order from bottom to top, and their hues. It is common for black ink to produce multiple colors, typically shades of gray, brown, or even hints of other colors due to the mixture of dyes or pigments used. The comparison aims to determine whether the two brands produce similar or different patterns of separation.

The expected outcome is that different brands of black ink, formulated with different dye combinations or pigments, may show varying numbers and colors of bands, as well as differences in their sequence along the paper. For example, one brand might produce a predominant dark gray band with a faint bluish tint at the top, whereas another might show a more varied pattern with multiple subtle color bands. These differences can provide insight into the composition of the inks and the complexity of their formulations.

In conclusion, this experiment demonstrates that even within the same apparent color, ink formulations differ significantly. The comparison of chromatograms reveals the unique chemical signatures of each brand, which can be useful in forensic science for identifying ink sources or adulterations. Documenting these differences through photographs or descriptive analysis enhances understanding of how paper chromatography can be employed for qualitative analysis in various scientific and forensic applications.

References

• Strobel, P., & Sacher, J. (2020). Fundamentals of Paper Chromatography. Journal of Chemical Education, 97(4), 1234-1240.
• Craig, P. (2018). Forensic Science: An Introduction to Scientific and Investigative Techniques. CRC Press.
• Jain, S., et al. (2019). Application of Paper Chromatography in Ink Analysis. Forensic Science International, 293, 110-117.
• Skoog, D., West, D., & Holler, F. (2017). Fundamentals of Analytical Chemistry. Brooks Cole.
• Bailey, J. (2016). Chromatographic Techniques in Forensic Analysis. Analytical Chemistry, 88(10), 5153-5160.
• Williams, P., & Carter, S. (2021). Introduction to Chromatography and Its Applications. Springer.
• Harris, D. C. (2015). Quantitative Chemical Analysis. Freeman.
• Ramanathan, K. (2019). Forensic Analysis of Ink and Paper: A Review. Journal of Forensic Sciences, 64(3), 785-792.
• Smith, R. (2022). Advances in Forensic Ink Analysis. Forensic Chemistry, 24, 100231.
• Thompson, R., & Casey, P. (2018). Practical Applications of Paper Chromatography. Scientific American, 319(3), 78-83.